To begin, transfer the diluted antibody solution into a special reagent bottle. Insert the detection kit into the reagent compartment of an automatic immunohistochemical standing instrument. Next, set the slides with the sectioned endometrial tissue on a slide holder and cover them with a special cover tile.
Then insert the holder into the experimental reaction compartment of the instrument. Once the instrument automatically recognizes the information on the reagent and slide, press the start button to initiate the immunohistochemical staining. After staining, take out the slide holder.
Put the stained slide into the slide holder to wash off any remaining dye with clean water. Now, place the slide holder onto an automated cover slipper and run the dehydration and sealing procedure. Remove the slides once dehydration and sealing are complete.
