Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma

1.8K Views

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06:20 min

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January 12th, 2024

DOI :

10.3791/65708-v

January 12th, 2024

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Noah A. Brown¹, Rajesh C. Rao¹^,²^,³^,⁴^,⁵^,⁶^,⁷^,⁸, Bryan L. Betz¹

¹Department of Pathology, University of Michigan, Ann Arbor, ²Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan, Ann Arbor, ³Department of Human Genetics, University of Michigan, Ann Arbor, ⁴Rogel Cancer Center, University of Michigan, Ann Arbor, ⁵Center of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, ⁶Center for RNA Biomedicine, University of Michigan, Ann Arbor, ⁷A. Alfred Taubman Medical Research Institute, University of Michigan, Ann Arbor, ⁸Section of Ophthalmology, Surgery Service, Veterans Administration Ann Arbor Healthcare System

Summary

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Cell free DNA Extraction

Vitreous Humor

Aqueous Humor

Vitreoretinal Lymphoma

Intact Neoplastic Cells

Chapters in this video

0:00

Introduction

1:36

Separation of Cellular and Cell‐Free Fractions of the Vitreous and Aqueous Humor for DNA Extraction

2:18

Extraction and Analysis of DNA from the Cellular Component of the Eye Humor

4:22

Cell‐Free DNA Extraction and Analysis from Eye Humor

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