Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
2.6K Views
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09:12 min
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April 5th, 2024
DOI :
April 5th, 2024
•Miguel M. Lopes*1,2,3, Sara M. Lopes*1,2,3, Rafael Baganha1,2,4, Carina Henriques1,2,5, Ana C. Silva1,2,3, Diana D. Lobo1,2,3, Luísa Cortes1,2,3,6, Luís Pereira de Almeida*1,2,4,5, Rui Jorge Nobre*1,2,3,4
1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra
Chapters in this video
0:00
Introduction
1:54
Production of Recombinant AAV by Transient Transfection
4:29
FPLC Purification and Characterization of Recombinant AAV
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