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JoVE Journal

Neuroscience

This content is Open Access.

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

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12:07 min

September 19th, 2010

September 19th, 2010

23,307 Views

0:00

Title

1:14

Introduction

1:47

Preparing the Animal for Imaging

4:46

Preparing the Thinned Skull Cortical Window

7:00

Two-photon Imaging

8:48

Injection of the HIV-1 Neurotoxin, Tat

11:37

Conclusion

We describe a method for repeatedly visualizing murine microglia and circulating monocytes in vivo over hours, days or weeks using transcranial two-photon microscopy. We demonstrate how to prepare a thinned-skull window that allows intermittent observation of quiescent microglia that can be activated by adjacent stereotactic injection of the HIV-1 regulatory protein Tat.

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