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Non-invasive Imaging of Leukocyte Homing and Migration in vivo

DOI :

10.3791/2062-v

December 5th, 2010

December 5th, 2010

12,534 Views

1Department of Pathology and Immunology, Washington University in St. Louis, 2National Institute of Neurological Disorders and Stroke, NINDS, NIH - National Institute of Health

Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.

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