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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

DOI :

10.3791/56218-v

October 30th, 2017

October 30th, 2017

6,843 Views

1Laboratory of Biochemical Genetics and Metabolism, The Rockefeller University

Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

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