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Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

DOI :

10.3791/2349-v

November 19th, 2010

November 19th, 2010

11,326 Views

1Department of Microbiology, Immunology and Pathology, Colorado State University

Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.

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