JoVE Logo
Faculty Resource Center

Sign In

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

DOI :

10.3791/60168-v

October 26th, 2019

October 26th, 2019

18,337 Views

1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 2The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 3Department of Oral Biology, University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba

Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.

Tags

Primary Culture

-- Views

Related Videos

article

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

article

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

article

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons

article

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

article

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

article

Primary Culture of Mouse Dopaminergic Neurons

article

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

article

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

article

Isolation and Culture of Embryonic Mouse Neural Stem Cells

article

Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved