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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

DOI :

10.3791/2461-v

February 1st, 2011

February 1st, 2011

14,930 Views

1Microbiology and Immunology, University of British Columbia - UBC

This protocol describes a high throughput screen for cellulolytic activity from a metagenomic library expressed in Escherichia coli. The screen is solution based and highly automated, and uses one-pot chemistry in 384 well microplates with the final readout as an absorbance measurement.

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High Throughput Screen

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