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Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

DOI :

10.3791/52509-v

April 20th, 2015

April 20th, 2015

17,420 Views

1Department of Physiology and Biophysics, Chicago Medical School

Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.

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Membrane Protein

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