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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung (Video) | JoVE

DOI :

10.3791/62985-v

November 16th, 2021

November 16th, 2021

4,970 Views

1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

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