Because the lung hosts a very complex and unique immune system, several gating strategies for identification of lung immune cells have been developed and reported. The existence of several different approaches makes it difficult to compare results
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In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.