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Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

DOI :

10.3791/3345-v

January 19th, 2012

January 19th, 2012

17,931 Views

1The Vollum Institute, Oregon Health and Science University

Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.

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Patch clamp Capacitance Measurements

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