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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

DOI :

10.3791/3709-v

10:58 min

June 28th, 2012

June 28th, 2012

16,076 Views

1Department of Molecular Biology and Biochemistry, University of California, Irvine, 2Department of Microbiology and Molecular Genetics, University of California, Irvine

Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.

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Hybridization In Situ

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