This content is Open Access.

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

24.4K Views

11:42 min

May 15th, 2012

May 15th, 2012


0:05

Title

1:55

Loading of First Bis-Peptide, Deprotection of First Bis-Peptide, and Simultaneous Resin Capping

3:43

Coupling Tert-butyl Carbamate (Boc)/tert-butyl Ester(tBu)-protected Functionalized Bis-Amino Acid

5:21

Deprotection of Boc/tBu-protected Functionalized Bis-Amino Acid, Deprotection of Fmoc, and Acylation of the First Bis-Amino Acid

6:55

Removal of the Boc Group from the Resin Bound Amino Acid and Cleavage from the Resin

7:56

Assessment Methods

9:51

Results: Analysis by Liquid Chromatography and Mass Spectroscopy

10:36

Conclusion

The efficient solid-phase peptide synthesis of a functionalized bis-peptide trimer utilizing a "safety catch" cleavage procedure from HMBA resin is described.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More