JoVE Logo
Faculty Resource Center

Sign In

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

DOI :

10.3791/50185-v

May 3rd, 2013

May 3rd, 2013

13,873 Views

1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München

We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.

-- Views

Related Videos

article

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

article

Establishing Primary Adult Fibroblast Cultures From Rodents

article

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

article

Primary Cell Cultures from Drosophila Gastrula Embryos

article

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

article

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

article

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

article

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

article

Isolation of Immune Cells from Primary Tumors

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved