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Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

DOI :

10.3791/50185-v

May 3rd, 2013

May 3rd, 2013

13,827 Views

1Department of Dermatology and Institute for Medical Engineering, Technische Universität München, 2Department of Dermatology and Allergology, Technische Universität München

We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.

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