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Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

DOI :

10.3791/51178-v

7:37 min

February 13th, 2014

February 13th, 2014

10,690 Views

1Microbial Biotechnology, Institute Biology Leiden, Leiden University, 2Microbiology, Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University

Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.

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