Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

12.2K Views

08:57 min

March 10th, 2014

DOI :

10.3791/51346-v

March 10th, 2014


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Pseudomonas Aeruginosa

Chapters in this video

0:05

Title

1:42

Preparation of Bacterial Strains and Biparental Conjugation

4:42

Detection and Isolation of Mucoid Colonies

6:00

gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis

7:07

Results: Mini-himar1 Mariner Transposon-mediated Mutagenesis

8:28

Conclusion

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