JoVE Logo
Faculty Resource Center

Sign In

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

DOI :

10.3791/51415-v

July 29th, 2014

July 29th, 2014

11,841 Views

1Institute for Virology, Philipps-University Marburg

Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.

-- Views

Related Videos

article

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

article

Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

article

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

article

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

article

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

article

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

article

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved