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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

DOI :

10.3791/59215-v

March 5th, 2019

March 5th, 2019

6,253 Views

1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

We present experimental approaches for studying RNA-interactors of double-stranded RNA binding protein kinase RNA-activated (PKR) during the mammalian cell cycle using HeLa cells. This method utilizes formaldehyde to crosslink RNA-PKR complexes and immunoprecipitation to enrich PKR-bound RNAs. These RNAs can be further analyzed through high-throughput sequencing or qRT-PCR.

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RNA Interactors

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