This protocol utilizes a widely-used technique along with reagents and tools that can be acquired commercially to assess endogenous IRF5 activation during the early events of stimulation. It can easily be applied for probing IRF5 biology in other
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A native Western blot method for analyzing endogenous interferon regulatory factor 5 dimerization in the CAL-1 plasmacytoid dendritic cell line is described. This protocol can be applied to other cell lines as well.