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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

46.0K Views

09:25 min

January 9th, 2015

DOI :

10.3791/52311-v

January 9th, 2015


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Keywords Cryo electron Microscopy

Chapters in this video

0:05

Title

1:21

Cryo-holder Preparation and Maintenance

2:32

Grid Preparation

3:48

Plunge-freezing

4:50

Transfer the Cryo-grid to the TEM

6:15

Low-dose Data Collection

7:03

Results: Visualization of Macromolecules Using Cryo-electron Microscopy

8:51

Conclusion

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