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Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

DOI :

10.3791/64322-v

6:26 min

January 6th, 2023

January 6th, 2023

1,545 Views

1Peak Proteins Ltd.

The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

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Fluorescent Size Exclusion Chromatography

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