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Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

10.8K Views

09:25 min

July 28th, 2015

DOI :

10.3791/52960-v

July 28th, 2015


Transcript

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Keywords Salmonella

Chapters in this video

0:05

Title

1:02

Preparation for Digitonin Assay with mCherry-expressing Salmonella

4:59

Infected Cells: FACS Analysis

6:03

Uninfected Permeabilization Controls: Microscopy

7:15

Results: Quantification of Cytosolic Salmonella Typhimurium

8:59

Conclusion

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