Results: Pheromone-induced Nueral Responses in GCaMP-expressing Nuerons
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Conclusion
Transcription
The overall goal of this procedure is to measure the physiological responses of Drosophila gustatory neurons to lipid pheromones. This technique can be used to answer key questions in sensory neurobiology such as identifying the ligands for orphan
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The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.