Activation of N9 Microglia With Lipopolysaccharide
5:02
Co-Cultivation of Neuronal PC12 Cells With N9 Microglia
7:31
Results: Lipopolysaccharide-activated N9 Microglia Secrete Cytokines That Are Toxic to Neuronal PC12 Cells
9:48
Conclusion
Transcript
The overall goal of this procedure is to create a cellular co-culture system of two cell types using inserts with a permeable membrane, thus allowing the diffusion of secreted soluble factors. This is accomplished with a series of steps that finel
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In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.