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Measuring Lactase Enzymatic Activity in the Teaching Lab

DOI :

10.3791/54377-v

4:41 min

August 6th, 2018

August 6th, 2018

103,222 Views

1School of Pharmacy, University of Reading, 2Department of Molecular Neuroscience, UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway, University of London

The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

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