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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

DOI :

10.3791/62431-v

9:03 min

July 19th, 2021

July 19th, 2021

3,205 Views

1Medical Mycology Research Center, Chiba University, 2Marine Works Japan Ltd.

Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.

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Rapid Freezing

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