Preparation and Administration of Adeno-Associated Virus via Subconjunctival Injection
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Results: Analysis of AAV Vector Administration, Biodistribution and Transgene Expression into the Subconjunctival Space
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Conclusion
Transcript
This protocol offers a method of gene therapy delivery to multiple ocular tissues in a safe, easy, and effective manner. It is also adaptable for intrastromal and subretinal injections in small animals. Use of the subconjunctival route of injectio
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In this manuscript, subconjunctival injection is demonstrated as a valid vector delivery method for ocular tissues in mice using an injection system consisting of an infusion/withdrawal syringe pump and a gastight removable syringe coupled with microinjection needles. This injection system is also adaptable for other intraocular administration routes.