Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
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The overall goal of these procedures is to adapt specific methodologies for the determination of mechanical properties such as, stiffness, viscosity and adhesion to the specific case of embryos and developing organisms. The methods that we introdu
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The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.