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Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

9.7K Views

08:53 min

May 7th, 2018

DOI :

10.3791/57293-v

May 7th, 2018


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Keywords Quantitative Analysis

Chapters in this video

0:05

Title

0:33

Preparation of Lipid Samples

3:06

Sample Preparation for Calibration Curve and Method Validation

3:50

SM Analysis by LC-ESI-MS/MS

5:33

Batch File Creation and Assigning the MS Three Product Ion Spectra of Sphingomyelin

6:23

Quantitative Analysis of SM by LC-ESI-MS/MS

7:02

Results: Validating the Quantitative and Qualitative Method for Sphingomyelin

8:01

Conclusion

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