This method can help answer key questions in the field of blood-brain barrier research, such as blood-brain barrier dysfunction, barrier leakage and signaling pathways that are specific for the human blood-brain barrier. The main advantage of this
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Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.