Isolation of Primary Pericytes from Bovine Brain Capillaries
3:01
Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
5:18
Live Imaging of Intracellular Ca2+ Levels
7:11
Results: Pericyte Isolation and Calcium Measurements
9:02
Conclusion
Transcript
This is a simple and effective method for isolating and culturing primary brain pericytes for study of intercellular calcium signaling. The obtained cell culture is a nearly homogenous population of pericytes, and it's straightforward to load the
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Brain capillary pericytes are essential players in the regulation of blood-brain barrier properties and blood flow. This protocol describes how brain capillary pericytes can be isolated, cultured, characterized with respect to cell type and applied for investigations of intracellular calcium signaling with fluorescent probes.