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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

DOI :

10.3791/57497-v

April 27th, 2018

April 27th, 2018

7,255 Views

1Department of Entomology and Plant Pathology, North Carolina State University

Here we present protocols for collecting and microinjecting precellular western corn rootworm embryos for the purpose of performing functional-genomic assays such as germline transformation and CRISPR/Cas9-genome editing.

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Microinjection

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