Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
5:31
Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
6:31
Conclusion
Transcript
The overall goal of this protocol is to visualize step-wise procedures involved in the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with chemical and physical agents of interest. Thi
Sign in or start your free trial to access this content
Vi beskriver en detaljert protokoll for Lambda Velg cII mutasjon analysen i kulturperler celler av transgene gnagere eller tilsvarende dyr behandlet med en kjemisk/fysisk agent av interesse. Denne tilnærmingen har vært mye brukt for genetisk virkning testing av kreftfremkallende faktorer i pattedyrceller.