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Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

DOI :

10.3791/57569-v

October 6th, 2018

October 6th, 2018

46,258 Views

1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital

Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.

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Keywords Dorsal Root Ganglia

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