Results: Transducing the Native Rodent Urothelium for Expression of Transgenes or Downregulation of Endogenous Gene Products
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Transcript
This protocol describes methods that allow investigators to express CDNAs or SIRNAs in the native bladder urothelium. The main advantages of this technique are its relative simplicity, the ability to express cDNAs, siRNAs in the urothelium with hi
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Methods are described for the generation of large amounts of recombinant adenoviruses, which can then be used to transduce the native rodent urothelium allowing for expression of transgenes or downregulation of endogenous gene products.