Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue

8.9K Views

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06:45 min

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January 19th, 2019

DOI :

10.3791/57952-v

January 19th, 2019

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Michael Chang¹^,², Suzie Dufour¹^,³, Peter L. Carlen¹^,²^,³^,⁵^,⁶, Taufik A. Valiante¹^,²^,³^,⁴

¹Division of Fundamental Neurobiology, Krembil Research Institute, ²Institute of Medical Science, Faculty of Medicine, University of Toronto, ³Institute of Biomaterials and Biomedical Engineering, University of Toronto, ⁴Division of Neurosurgery, Department of Surgery, University of Toronto, ⁵Division of Neurology, Department of Medicine, University of Toronto, ⁶Department of Physiology, University of Toronto

Summary

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Keywords Acute Ictal Activity

Seizure Mechanisms

Electrographic Seizure

Anti seizure Therapies

Electrophysiological Recordings

Chapters in this video

0:04

Title

0:46

Preparation of Cortical Slices from the Somatosensory-motor Cortex

2:40

Electrophysiological Recordings of the Superficial Cortical Layer and Seizure Induction

4:43

Results: Acute In Vitro 4-AP Cortical Seizure Model and Ictal Events Generated by Optogenetics

6:19

Conclusion

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