Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
9.0K Views
•
09:21 min
•
October 22nd, 2018
DOI :
October 22nd, 2018
•Chapters in this video
0:04
Title
0:41
Cell Culturing
1:37
4tU Labeling with S. pombe as a Spike-in
3:20
RNA Extraction and DNase Treatment
4:30
Thiol-specific Biotinylation of Newly Synthesized RNA
6:05
Purification of Newly Synthesized Fraction from Total and Unlabeled RNA Using Streptavidin-coated Magnetic Beads
7:18
Results: Quantification of Newly Synthesized mRNA as a Proxy for RNA Polymerase II Activity
8:52
Conclusion
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