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Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

DOI :

10.3791/58206-v

7:37 min

October 11th, 2018

October 11th, 2018

8,845 Views

1Anesthesiology & Perioperative Medicine, Oregon Health & Science University, 2Environmental and Biological Services Division, Pacific Northwest National Laboratory, 3Operative Care Division, Portland Veterans Affairs Medical Center

We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.

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Micropuncture

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