Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

8.9K Views

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08:50 min

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October 21st, 2018

DOI :

10.3791/58478-v

October 21st, 2018

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H.S. Jeffrey Man¹^,², Aravin N. Sukumar¹^,², Kyung Ha Ku²^,³, Michelle K. Dubinsky¹^,², Noeline Subramaniam¹^,², Philip A. Marsden¹^,²^,³^,⁴

¹Institute of Medical Science, University of Toronto, ²Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, ³Department of Laboratory Medicine and Pathobiology, University of Toronto, ⁴Department of Medicine, University of Toronto

Summary

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Keywords Endothelial Cells

Shear Stress

Parallel plate Flow Chambers

Gene Expression Analysis

Chapters in this video

0:04

Title

0:55

Assay Setup

4:20

Flow Assay

5:18

Cell Harvest and RNA Extraction

6:48

Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress

8:06

Conclusion

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