Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
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Conclusion
필기록
This method can help answer key questions in the field of endothelial biology, such as why do blood vessels have regional differences in the development of atherosclerosis? The main advantage of this technique is it allows the simultaneous study o
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Here, a workflow for the culture and gene expression analysis of endothelial cells under fluid shear stress is presented. Included is a physical arrangement for simultaneously housing and monitoring multiple flow chambers in a controlled environment and the use of an exogenous reference RNA for quantitative PCR.