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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

DOI :

10.3791/58589-v

5:30 min

January 23rd, 2019

January 23rd, 2019

12,605 Views

1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz

This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

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Methanol Independent Expression

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