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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

DOI :

10.3791/58612-v

October 25th, 2018

October 25th, 2018

8,227 Views

1Center for Food Safety

Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples. 

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