JoVE Logo

Sign In

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

21.6K Views

00:09 min

September 4th, 2019

DOI :

10.3791/59780-v

September 4th, 2019


Explore More Videos

CRISPR

Chapters in this video

0:04

Title

1:36

Pooled CRISPR sgRNA Lentiviral Library Plasmid Amplification

2:24

Large-scale CRISPR sgRNA Library Lentivirus Production

3:47

Primary Screen Infection, Selection, and Cell Passaging

5:41

CRISPR Sample Preparation and Sequencing

6:43

Results: Genome-scale CRISPR Screening

7:54

Conclusion

Related Videos

article

11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

12.4K Views

article

11:15

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

7.2K Views

article

08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

9.0K Views

article

11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

9.4K Views

article

08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

14.4K Views

article

07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

6.3K Views

article

14:51

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

3.2K Views

article

09:33

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

1.1K Views

article

07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

90 Views

article

09:12

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

18.6K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved