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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

DOI :

10.3791/58911-v

May 12th, 2019

May 12th, 2019

7,083 Views

1Graduate School of Medicine and Faculty of Medicine, Kyoto University, 2Graduate School of Informatics, Kyoto University

This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).

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3D Scanning Technology

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