Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

14.5K Views

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10:50 min

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May 1st, 2019

DOI :

10.3791/58947-v

May 1st, 2019

•

Sai Rashmika Velugula-Yellela¹, David N. Powers¹, Phillip Angart¹, Anneliese Faustino¹, Talia Faison¹, Casey Kohnhorst¹, Erica J. Fratz-Berilla¹, Cyrus D. Agarabi¹

¹Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

Summary

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Monoclonal Antibody

Chinese Hamster Ovary Cells

Microbioreactor System

Purification System

Critical Quality Attributes

Liquid Chromatography Mass Spectrometry LCMS

Enzyme friendly Surfactant

Protein Concentration

Sample Injection

Chapters in this video

0:04

Title

0:39

Antibody Purification

1:49

Purified Antibody Concentration

2:33

N-Glycan Labeling and Isolation

5:00

Labeled N-Glycan Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis

6:17

Charge Variant Sample Preparation

7:38

Charge Variant Chip Preparation

8:31

Results: Representative Purified Monoclonal Antibody Automated Microbioreactor System Analyses

9:58

Conclusion

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