Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

11.8K Views

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07:26 min

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May 19th, 2019

DOI :

10.3791/59652-v

May 19th, 2019

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Zachary J. Heins¹^,², Christopher P. Mancuso¹^,², Szilvia Kiriakov¹^,³, Brandon G. Wong¹^,², Caleb J. Bashor⁴, Ahmad S. Khalil¹^,²^,⁵

¹Biological Design Center, Boston University, ²Department of Biomedical Engineering, Boston University, ³Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, ⁴Department of Bioengineering, Rice University, ⁵Wyss Institute for Biologically Inspired Engineering, Harvard University

Summary

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EVOLVER

Automated Experiments

Continuous Cell Growth

High throughput Culture

Aerobic Culturing

Anaerobic Fermentation

Sterilization Process

Fluid Lines

Waste Management

Touchscreen Navigation

Chapters in this video

0:04

Title

0:58

Sterilizing the System

2:56

Loading the Culture Vials

3:51

Configuring eVOLVER Software and Programming Algorithmic Culture Routines

4:48

Inoculating and Monitoring Experiment in Real-time

5:47

Results: Fitness Surface Construction

6:35

Conclusion

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