Results: Representative IgG Uptake Staining in a Degenerating Mdx Muscle Cross-Section
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Conclusion
Transcript
Myofiber necrosis is characteristic of multiple neuromuscular disorders and plays a central role in pathogenesis. A specific, reliable method for assessing muscle degeneration is important for diagnosis and translational research. This extremely a
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Described here is a protocol for direct immunolabelling of necrotic myofibers in muscle cryosections. Necrotic cells are permeable to serum proteins, including immunoglobulin G (IgG). Revealing the uptake of IgG by myofibers allows the identification and quantification of myofibers that undergo necrosis regardless of muscle condition.