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Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo

DOI :

10.3791/59800-v

August 31st, 2019

August 31st, 2019

8,530 Views

1Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 2Academy of Scientific and Innovative Research (AcSIR)

Presented here is a protocol for the spontaneous generation of neurospheres enriched in neural progenitor cells from high density plated neurons. During the same experiment, when neurons are plated at a lower density, the protocol also results in prolonged primary rat neuron cultures.

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Neurospheres

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