October 4th, 2019
•Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.
Tags
Related Videos
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved